Polyclonal Anti-CHEK1

Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase Chk1 (CHEK1) detection. Tested with WB in Human;Mouse;Rat. \n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1445

Size

100ug/vial

Applications

WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

CHEK1

References

1. Flaggs, G., Plug, A. W., Dunks, K. M., Mundt, K. E., Ford, J. C., Quiggle, M. R. E., Taylor, E. M., Westphal, C. H., Ashley, T., Hoekstra, M. F., Carr, A. M. Atm-dependent interactions of a mammalian Chk1 homolog with meiotic chromosomes. Curr. Biol. 7: 977-986, 1997.\n2. Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H., Elledge, S. J. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-1501, 1997.\n3. Zhang, Y.-W., Otterness, D. M., Chiang, G. G., Xie, W., Liu, Y.-C., Mercurio, F., Abraham, R. T. Genotoxic stress targets human Chk1 for degradation by the ubiquitin-proteasome pathway. Molec. Cell 19: 607-618, 2005.\n

Swiss Prot

O14757

Storage Temp

\"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n\"\n

Additional Info

A synthetic peptide corresponding to a sequence at the N-terminal of human CHEK1, identical to the related mouse and rat sequence.

Scientific Background

CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization,the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.