Polyclonal Anti-COL4A1

Price

200.00 USD

Size

100?g/vial

Reactivities

Hum

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Mayne, R., Wiedemann, H., Irwin, M. H., Sanderson, R. D., Fitch, J. M., Linsenmayer, T. F., Kuhn, K.Monoclonal antibodies against chicken type IV and V collagens: electron microscopic mapping of the epitopes after rotary shadowing.J. Cell Biol. 98: 1637-1644, 1984.\n2. Solomon, E., Hiorns, L. R., Spurr, N., Kurkinen, M., Barlow, D., Hogan, B. L. M., Dalgleish, R.Chromosomal assignments of the genes coding for human types II, III and IV collagen: a dispersed gene family.Proc. Nat. Acad. Sci. 82: 3330-3334, 1985.\n3. Sudhakar, A., Nyberg, P., Keshamouni, V. G., Mannam, A. P., Li, J., Sugimoto, H., Cosgrove, D., Kalluri, R.Human alpha-1 type IV collagen NC1 domain exhibits distinct antiangiogenic activity mediated by alpha-1-beta-1 integrin.J. Clin. Invest. 115: 2801-2810, 2005.\n

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n

Scientific Background

Collagen alpha-1(IV) chain is a protein that in humans is encoded by the COL4A1 gene. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in COL4A1 exons 24 and 25 are associated with HANAC (autosomal dominant hereditary angiopathy with nephropathy,aneurysms, and muscle cramps).