| Catalogue number: |
PA1909 |
| Price: |
$200.00 |
| Reactivities: |
Mouse, Rat |
| Applications: |
Immunohistochemistry, Western Blot |
| Size: |
100ug/vial |
| Gene: |
CYP2E1 |
| Swiss prot: |
Q05421 |
| Form: |
Lyophilized |
| Format: |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: |
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: |
CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans?and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful. |
| References: |
1. Nebert, D. W.; Adesnik, M.; Coon, M. J.; Estabrook, R. W.; Gonzalez, F. J.; Guengerich, F. P.; Gunsalus, I. C.; Johnson, E. F.; Kemper, B.; Levin, W.; Phillips, I. R.; Sato, R.; Waterman, M. R. : The P450 gene superfamily: recommended nomenclature. DNA 6: 1-11, 1987.
2. Song, B.-J.; Gelboin, H. V.; Park, S.-S.; Yang, C. S.; Gonzalez, F. J. : Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. J. Biol. Chem. 261: 16689-16697, 1987.
3. Hayashi, S.; Watanabe, J.; Kawajiri, K. : Genetic polymorphisms in the 5-prime-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110: 559-565, 1991.
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| Additional info: |
A synthetic peptide corresponding to a sequence at the N-terminal of mouse CYP2E1, identical to the related rat sequence. |
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