| Catalogue number: |
PA1030 |
| Price: |
$200.00 |
| Reactivities: |
Human, Mouse, Rat |
| Applications: |
Immunohistochemistry, Western Blot |
| Size: |
100ug/vial |
| Gene: |
DUT |
| Swiss prot: |
P33316 |
| Form: |
Lyophilized |
| Format: |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: |
"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
"
|
| Scientific background: |
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E. coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis. |
| References: |
1. Studebaker AW, Lafuse WP, Kloesel R, Williams MV.Modulation of human dUTPase using small interfering RNA. Biochem Biophys Res Commun. 2005 Feb 4; 327(1):306-10.
2. McIntosh EM, Ager DD, Gadsden MH, Haynes RH.Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc Natl Acad Sci U S A. 1992 Sep 1; 89(17):8020-4.
3. Chu R, Lin Y, Rao MS, Reddy JK.Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha. J Biol Chem. 1996 Nov 1; 271(44):27670-6.
3. Ladner RD, Caradonna SJ.The human dUTPase gene encodes both nuclear and mitochondrial isoforms.Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. J Biol Chem. 1997 Jul 25; 272(30):19072-80.
4. Ladner RD.The role of dUTPase and uracil-DNA repair in cancer chemotherapy. Curr Protein Pept Sci. 2001 Dec; 2(4):361-70. |
| Additional info: |
A synthetic peptide corresponding to a sequence in the middle region of human dUTPase(136-150aa YDLYSAYDYTIPPME), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids. |
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