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Products Polyclonal Anti-E-Selectin
| Catalogue number: | PA1704 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Immunohistochemistry, Western Blot |
| Size: | 100ug/vial |
| Gene: | SELE |
| Swiss prot: | P16581 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | E-Selectin(SELE), also called Selectin E, ELAM1 or ELAM, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. It is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. E-Selection is a member of the selectin family of cell adhesion molecules. The E-Selectin gene is mapped on 1q24.2. The ELAM gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA by Collins et al. Using affinity chromatography, pull-down assays, and mass spectrometric analysis on adenocarcinoma cell lines, Gout et al. showed that SELE bound death receptor-3 on the cancer cells. Western blot analysis revealed that the SELE-binding protein was recognized by anti-DR3 antibodies. Anti-DR3 or knockdown of DR3 by small interfering RNA decreased adhesion of colon cancer cells to SELE and to SELE-expressing endothelial cells. |
| References: | 1.Bevilacqua, M. P., Stengelin, S., Gimbrone, M. A., Jr., Seed, B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243: 1160-1165, 1989. 2.Chang, Y.-P. C., Liu, X., Kim, J. D. O., Ikeda, M. A., Layton, M. R., Weder, A. B., Cooper, R. S., Kardia, S. L. R., Rao, D. C., Hunt, S. C., Luke, A., Boerwinkle, E., Chakravarti, A. Multiple genes for essential-hypertension susceptibility on chromosome 1q. Am. J. Hum. Genet. 80: 253-264, 2007. 3.DeLisser, H. M., Christofidou-Solomidou, M., Sun, J., Nakada, M. T., Sullivan, K. E. Loss of endothelial surface expression of E-selectin in a patient with recurrent infections. Blood 94: 884-894, 1999. |
| Additional info: | A synthetic peptide corresponding to a sequence at the N-terminal of human E-Selectin, different from the related rat and mouse sequences by two amino acids. |
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