Polyclonal Anti-GLI3

Rabbit IgG polyclonal antibody for Transcriptional activator GLI3 (GLI3) detection. Tested with WB in Human;Mouse;Rat. \n\n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1766

Size

100ug/vial

Applications

WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

GLI3

References

1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early \nstages of limb development. (Letter) Nature 436: 277-281, 2005.\n2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. \nMolec. Genet. 11: 1129-1135, 2002.\n3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan, \nV., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in \nhypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

Swiss Prot

P10071

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence at the N-terminal of \nhuman GLI3, different from the related rat sequence by two amino \nacids, and from the related mouse sequence by one amino acid.

Scientific Background

GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc \nfinger gene family related to Kruppel, a gene that is known to regulate development in Drosophila.\nThe GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and \nlung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. \nThe GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) \ngene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) \ngenes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor \nin a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog \nprotein. The high relative abundance and potency of GLI3 repressor suggested specialization \nof GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and \nimmunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on \nproteasome activity. The findings suggested that BTRC is required for GLI3 processing.