Polyclonal Anti-Gremlin 1

Rabbit IgG polyclonal antibody for Gremlin-1 (GREM1) detection. Tested with WB in Human;Mouse;Rat. \n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1319

Size

100ug/vial

Applications

WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

GREM1

References

1. Gazzero E, Pereira RC, Jorgetti V, Olson S, Economides AN, Canalis E (2005). \"Skeletal overexpression of gremlin impairs bone formation and causes osteopenia\". Endocrinology 142(2):655-665. \n2. Stabile H, Mitola S, Moroni E, Belleri M, Nicoli S, Coltrini D, Peri F, Pessi A, Orsatti L, Talamo F (2007). Blood 109(5):1834-1840. \n3. Gazzero E, Pereira RC, Jorgetti V, Olson S, Economides AN, Canalis E (2005). \"Skeletal overexpression of gremlin impairs bone formation and causes osteopenia\". Endocrinology 142(2), 146(2):655-665. \n4. Namkoong H, Shin SM, Kim HK, Ha S, Cho GW, Hur SY, Kim TE, Kim JW (2006). \"The bone morphognentic protein antagonist gremlin 1 is overespressed in human cancers and interacts with YWAHAH protein\". BMC cancer 6:74.

Swiss Prot

O60565

Storage Temp

\"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n\"\n

Additional Info

A synthetic peptide corresponding to a sequence at the C-terminal of human Gremlin 1, identical to the related rat and mouse sequences.

Scientific Background

Gremlin, also known as Drm, is a highly conserved 20.7-kDa, 184 amino acid glycoprotein part of the DAN family and is a cysteine knot-secreted protein. Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. And Gremlin is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. Gremlin 1 (GREM 1) is known for its antagonistic reaction with BMPs in the TGF beta signaling pathway. This gene inhibits BMP-2, BMP-4, and BMP-7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. Gremlin 1 may play an oncogenic role especially in carcinomas of the uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma. Over-expressed gremlin 1 functions by interaction with YWHAH(Its binding site for gremlin 1 was located between residues 61-80 and gremlin 1 binding site for YWHAH was found to be located between residues 1-67). Therefore, Gremlin 1 and its binding protein YWHAH could be good targets for developing diagnostic and therapeutic strategies against human cancers.