Polyclonal Anti-INSL3

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Adham IM, Burkhardt E, Benahmed M, Engel W.Cloning of a cDNA for a novel insulin-like peptide of the testicular Leydig cells. J Biol Chem. 1993 Dec 15; 268(35):26668-72.\n2. Burkhardt E, Adham IM, Brosig B, Gastmann A, Mattei MG, Engel W.Structural organization of the porcine and human genes coding for a Leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3). Genomics. 1994 Mar 1; 20(1):13-9.\n3. Zimmermann S, Steding G, Emmen JM, Brinkmann AO, Nayernia K, Holstein AF, Engel W, Adham IM.Targeted disruption of the Insl3 gene causes bilateral cryptorchidism. Mol Endocrinol. 1999 May; 13(5):681-91.\n4. Foresta C, Ferlin A.Role of INSL3 and LGR8 in cryptorchidism and testicular functions. Reprod Biomed Online. 2004 Sep; 9(3):294-8.

Storage Temp

\"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n\"\n

Scientific Background

Insulin-like 3 (INSL3), a member of the insulin-like hormone superfamily, is specifically expressed in Leydig cells of the fetal and postnatal testis and in theca cells of the postnatal ovary. It is synthesized as a 131-amino acid preproprotein, which contains a 24-amino acid signal peptide. The human INSL3 gene is assigned to bands p13.2-p12 of the short arm of chromosome 19 with the similar organization to that of insulin and relaxin. INSL3 induces gubernaculum development in an androgen-independent way, while androgen-mediated regression of the CSL occurs independently from Insl3. Moreover, INSL3 is a ligand for LGR8 and INSL3-LGR8 mutations are believed to be associated with human cryptorchidism.