

Supplier:
BOSTER IMMUNOLEADERCat no: PA1793
Polyclonal Anti-NOL3
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SPECIFICATIONS
Price
200.00 USD
Catalog Number
PA1793
Size
100ug/vial
Applications
WB
Reactivities
Hum, Mouse, Rat
Form
Lyophilized
Format
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Gene Id
NOL3
References
1. Abmayr, S., Crawford, R. W., Chamberlain, J. S.Characterization of ARC, apoptosis repressor \ninteracting with CARD, in normal and dystrophin-deficient skeletal muscle.Hum. Molec. Genet. 13: \n213-221, 2004.\n2. Koseki, T., Inohara, N., Chen, S., Nunez, G.ARC, an inhibitor of apoptosis expressed in skeletal \nmuscle and heart that interacts selectively with caspases.Proc. Nat. Acad. Sci. 95: 5156-5160, 1998.\n3. Stoss, O., Schwaiger, F.-W., Cooper, T. A., Stamm, S.Alternative splicing determines the intracellular \nlocalization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c.J. Biol. \nChem. 274: 10951-10962, 1999.
Swiss Prot
O60936
Storage Temp
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.
Additional Info
A synthetic peptide corresponding to a sequence in the middle region of \nhuman NOL3, identical to the related rat sequence?different from the related rat and mouse sequences by \none amino acid.
Scientific Background
NOL3(Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is \nencoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic \nsequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP \nand which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates \nthe 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller \ntranscript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when \ncoexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor \nnecrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits \nthe enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC \ninteracts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with \nCASP1, CASP3, or CASP9.
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