| Catalogue number: |
PA1670 |
| Price: |
$200.00 |
| Reactivities: |
Human, Mouse, Rat |
| Applications: |
Immunohistochemistry, Western Blot |
| Size: |
100ug/vial |
| Gene: |
SLC10A1 |
| Swiss prot: |
Q14973 |
| Form: |
Lyophilized |
| Format: |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: |
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: |
SLC10A1(SOLUTE CARRIER FAMILY 10 (SODIUM/BILE ACID COTRANSPORTER FAMILY), MEMBER 1),also called NTCP or NTCP1,is found in the basolateral membranes of hepatocytes, which is also involved in the reabsorption of bile acids. The SLC10A1 gene is mapped on 14q24.2.The SLC10A1 gene contains 5 exons and spans about 23 kb. Like other sodium/bile acid transporters, SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane.Injection of in vitro transcribed NTCP cRNA into Xenopus oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. NTCP-mediated taurocholate uptake in oocytes was inhibited by all major bile acid derivatives and by bromosulfophthalein. |
| References: |
1. Green, R. M., Ananthanarayanan, M., Suchy, F. J., Beier, D. R. Genetic mapping of the Na(+)-taurocholate cotransporting polypeptide to mouse chromosome 12. Mammalian Genome 9: 598-600, 1998.
2. Hagenbuch, B., Meier, P. J. Molecular cloning, chromosomal localization, and functional characterization of a human liver Na(+)/bile acid cotransporter. J. Clin. Invest. 93: 1326-1331, 1994.
3. Hallen, S., Mareninova, O., Branden, M., Sachs, G. Organization of the membrane domain of the human liver sodium/bile acid cotransporter. Biochemistry 41: 7253-7266, 2002.
|
| Additional info: |
A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1, different from the related rat and mouse sequences by one amino acid. |
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