Polyclonal Anti-SLC6A1

Rabbit IgG polyclonal antibody for Sodium- and chloride-dependent GABA transporter 1 (SLC6A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. \n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1721

Size

100ug/vial

Applications

IHC, WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

SLC6A1

References

1.Cope, D. W., Di Giovanni, G., Fyson, S. J., Orban, G., Errington, A. C., Lorincz, M. L., Gould, T. M., Carter, D. A., Crunelli, V. Enhanced tonic GABA-A inhibition in typical absence epilepsy. Nature Med. 15: 1392-1398, 2009. \n2.Huang, F., Shi, L. J., Heng, H. H. Q., Fei, J., Guo, L.-H. Assignment of the human GABA transporter gene (GABATHG) locus to chromosome 3p24-p25. Genomics 29: 302-304, 1995. \n3.Zomot, E., Bendahan, A., Quick, M., Zhao, Y., Javitch, J. A., Kanner, B. I. Mechanism of chloride interaction with neurotransmitter:sodium symporters. Nature 449: 726-730, 2007.\n

Swiss Prot

P30531

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence in the middle region of human SLC6A1, identical to the related rat and mouse sequences.

Scientific Background

SLC6A1(Solute Carrier family 6 (Neurotransmitter transporter, Gaba), Member 1), also called GABATR or GABATHG, encodes a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft. The SLC6A1 gene contains 15 exons and is approximately 25 kb by Lam et al. And it is mapped on 3p25.3. Zomot et al. (2007) showed that introduction of a negatively charged amino acid at or near 1 of the 2 putative sodium binding sites of the GABA transporter GAT1 from rat brain (also calledSLC6A1) renders both net flux and exchange of GABA largely chloride independent. By in vitro functional expression studies, the SLC6A1 insertion promoter (2 copies of the sequence) had significantly greater promoter activity than the noninsertion (1 copy of the sequence) promoter, which in turn had greater activity than a promoterless control. Studies of postmortem hippocampus from 69 African American individuals showed that the SLC6A1promoter genotype predicted SLC6A1 RNA expression and that overall SLC6A1 expression decreased with age.