Polyclonal Anti-SMAD2/3

Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2/3 (SMAD2/3) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. \n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1073-1

Size

100ug/vial

Applications

IHC, IHC-Fr, WB

Reactivities

Hum, Mouse, Rat

Form

Liquid

Format

Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4.

Gene Id

SMAD2/3

References

1. Riggins G.J., Thiagalingam S., Rosenblum E., Weinstein C.L., Kern S.E., Hamilton S.R., Willson J.K.V., Markowitz S.D., Kinzler K.W., Vogelstein B.V.;\"Mad-related genes in the human.\";Nat. Genet. 13:347-349(1996).\n2. Zhang Y., Feng X.-H., Wu R.-Y., Derynck R.;\"Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.\";Nature 383:168-172(1996).\n3. Inman, G. J.; Nicolas, F. J.; Hill, C. S. : Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Molec. Cell 10: 283-294, 2002.

Swiss Prot

Q15796

Storage Temp

\"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n\"\n

Additional Info

A synthetic peptide corresponding to the N-terminal of human SMAD2/3, identical to the related mouse and rat sequences.

Scientific Background

SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Transforming growth factor (TGF)-beta stimulation leads to phosphorylation and activation of Smad2 and Smad3, which form complexes with Smad4 that accumulate in the nucleus and regulate transcription of target genes. Smad2 and Smad3 share highly homology. SMAD2/SMAD3 signal transduction appeared to be important in the regulation of muscle-specific genes. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development.Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development.