Polyclonal Anti-SNAP23

Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 23 (SNAP23) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. \n\n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1774

Size

100ug/vial

Applications

IHC, IHC-Fr, WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

SNAP23

References

1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell \nsurface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998.\n2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F., \nEstivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the \nhuman synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion \nprocesses. Hum. Genet. 108: 211-215, 2001.\n3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein \nSNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812, \n1997.

Swiss Prot

O00161

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence at the C-terminal of \nhuman SNAP23, different from the related rat and mouse sequences\nby three amino acids .

Scientific Background

SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans \nis encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in \nexon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino \nacid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of \nSNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to \nbind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After \nrelocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. \nTIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A \nand SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl \nmaleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and \nfunctionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is \nan essential component of the high affinity receptor for the general membrane fusion machinery and is \nan important regulator of transport vesicle docking and fusion.