Polyclonal Anti-Stat3

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Akira, S., Nishio, Y., Inoue, M., Wang, X.-J., Wei, S., Matsusaka, T., Yoshida, K., Sudo, T., Naruto, \nM., Kishimoto, T. Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related \ntranscription factor involved in the gp130-mediated signaling pathway. Cell 77: 63-71, 1994.\n2. Bhattacharya, S., Schindler, C. Regulation of Stat3 nuclear export. J. Clin. Invest. 111: 553-559, \n2003.\n3. Bong, Y.-S., Lee, H.-S., Carim-Todd, L., Mood, K., Nishanian, T. G., Tessarollo, L., Daar, I. \nO. ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3. Proc. Nat. Acad. \nSci. 104: 17305-17310, 2007.

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription \nfactor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member \nof the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor \nactivates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression \nof STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a \nvariety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such \nas cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at \nits amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl \nterminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell \nlines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for \ntransformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered \nglycolytic and oxidative phosphorylation activities characteristic of cancer cells.