Polyclonal Anti-TNFR1

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Fuchs, P.; Strehl, S.; Dworzak, M.; Himmler, A.; Ambros, P. F. : Structure of the human TNF receptor 1 (p60) gene (TNFR1) and localization to chromosome 12p13. Genomics 13: 219-224, 1992.\n2. Hohmann, H.-P.; Remy, R.; Brockhaus, M.; van Loon, A. P. G. M. : Two different cell types have different major receptors for human tumor necrosis factor (TNF-alpha). J. Biol. Chem. 264: 14927-14934, 1989. \n3. Schievella, A. R.; Chen, J. H.; Graham, J. R.; Lin, L.-L. : MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J. Biol. Chem. 272: 12069-12075, 1997. \n4. Micheau, O.; Tschopp, J. : Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes. Cell 114: 181-190, 2003.

Storage Temp

\"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n\"\n

Scientific Background

Tumor necrosis factor receptor 1(TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.