Polyclonal Anti-Vinculin/VCL

Rabbit IgG polyclonal antibody for Vinculin (VCL) detection. Tested with WB, IHC-P in Human;Mouse;Rat. \n\n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1781

Size

100ug/vial

Applications

IHC, WB

Reactivities

Mouse

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

VCL

References

1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley, \nD. R., Craig, S. W., Liddington, R. C.Structural basis for vinculin activation at sites of cell \nadhesion.Nature 430: 583-586, 2004.\n2. Mulligan, L. M., Gardner, E., Telenius, H., Ponder, B. A. J.Complementary physical and genetic \ntechniques map the vinculin (VCL) gene on chromosome 10q.Genomics 13: 1347-1349, 1992.\n3. Vasile, V. C., Edwards, W. D., Ommen, S. R., Ackerman, M. J.Obstructive hypertrophic \ncardiomyopathy is associated with reduced expression of vinculin in the intercalated disc.Biochem. \nBiophys. Res. Commun. 349: 709-715, 2006.

Swiss Prot

P18206

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence at the N-terminal of \nhuman VCL, identical to the related rat and mouse sequences.

Scientific Background

In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is \ninvolved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a \n3-generation family, Mulligan et al. (1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to \nD10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is \nthought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. \nMultiple alternatively-spliced transcript variants have been found for this gene, but the biological validity \nof some variants has not been determined. Human vinculin protein exhibits a greater-than-95% \nsequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 \namino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain \nseparated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains \nbinding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region \ncontains binding sites for F-actin, paxillin, and lipids.