Ponasterone A (Pon A, 25-Deoxy-20-hydroxyecdysone, 25-Deoxyecdysterone, AI3-44686, 2beta,3beta,14alpha,20R,22R-Pentahydroxy-5beta-cholest-7-en-6-one)

Ponasterone A is a member of the ecdysteroid family. Ecdysone receptor (EcR) agonist. An analog of ecdysone with similar properties to muristerone A. Induces expression of b-galactosidase to levels similar to those obtained with muristerone A induction. Potent compound for ecdysteroid activity. Insect steroid hormone involved in regulating metamorphosis.\n\nSolubility:\nDMSO, EtOH\n\nMolecular Weight:\n~464.63\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

045162

Size

1mg

Purity

>95% (HPLC)

References

Product Reference: \n?Extraction of ponasterone A and ecdysterone from podocarpaceae and related plants: S. Imai et al.; Steroids 10, 557 (1967)\n?Ecdysones and analogs: effects on development and reproduction of insects: W.E. Robbins, et al.; Science 161, 1158 (1968)\n?The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol. 17, 51 (1980)\n?Past and present studies with ponasterones, the first insect molting hormones from plants: K. Nakanishi; Steroids 57, 649 (1992)\n?Identification of ligands and coligands for the ecdysone-regulated gene switch: E. Saez, et al.; PNAS 97, 14512 (2000)\n?Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells: C. Rampazzo, et al.; J. Biol. Chem. 275, 5409 (2000)\n?Sustained mammary gland-directed, ponasterone A-inducible expression in transgenic mice: C. Albanese, et al.; FASEB J. 14, 877 (2000)\n?The ecdysone inducible gene expression system: unexpected effects of muristerone A and ponasterone A on cytokine signaling in mammalian cells: S. Constantino, et al.; Eur. Cytokine Netw. 12, 365 (2001)\n?Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol. 13, 129 (2002)\n?Potent and selective partial ecdysone agonist activity of chromafenozide in Sf9 cells: T. Toya, et al.; BBRC 292, 1087 (2002)\n?Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol. 35, 79 (2003)\n?Development of an in vitro Reporter Gene Assay for Screening the Ecdysone Receptor Agonists in Mysid Crustacean: M. Hirano, et al.; Terrapub 141 (2010)\n?\n