Home  >  Products  >  Pou5f1 (POU Domain, Class 5, Transcription Factor 1, NF-A3, Octamer-binding Protein 3, Oct-3, Octamer-binding Protein 4, Oct-4, Octamer-binding Transcription Factor 3, OTF-3, Oct-3, Oct-4, Otf-3, Otf3)
Pou5f1 (POU Domain, Class 5, Transcription Factor 1, NF-A3, Octamer-binding Protein 3, Oct-3, Octamer-binding Protein 4, Oct-4, Octamer-binding Transcription Factor 3, OTF-3, Oct-3, Oct-4, Otf-3, Otf3)

Pou5f1 (POU Domain, Class 5, Transcription Factor 1, NF-A3, Octamer-binding Protein 3, Oct-3, Octamer-binding Protein 4, Oct-4, Octamer-binding Transcription Factor 3, OTF-3, Oct-3, Oct-4, Otf-3, Otf3)

Cat no: O5403-01X

Supplier: United States Biological
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Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml, observed in lysates of cell line NIH3T3 on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: O5403-01X
Reactivities: Mouse, Rat
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes mouse OCT4. Species sequence homology: Rat.

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