PRDX2, Recombinant, Human (Peroxiredoxin-2, Thioredoxin Peroxidase 1, Thioredoxin-dependent Peroxide Reductase 1, Thiol-specific Antioxidant Protein, TSA, PRP, Natural Killer Cell-enhancing Factor B, NKEF-B, TDPX1, NKEFB)

Supplier:

United States Biological

Cat no: P3352-12P

PRDX2, Recombinant, Human (Peroxiredoxin-2, Thioredoxin Peroxidase 1, Thioredoxin-dependent Peroxide Reductase 1, Thiol-specific Antioxidant Protein, TSA, PRP, Natural Killer Cell-enhancing Factor B, NKEF-B, TDPX1, NKEFB)

Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the aa sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30kD in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol (1).\n\nSource:\nRecombinant corresponding to aa1-198, from human Peroxiredoxin II, expressed in E.coli.\n\nMolecular Weight:\n~22kD\n\nSpecific Activity:\n2.2U/mg (One unit will cause the oxidation of 1umole of NADPH at 25 degrees C at pH 7.5)\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

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SPECIFICATIONS

Catalog Number

P3352-12P

Size

200ug

Form

Supplied as a liquid in 20mM HEPES, pH 7.0, 10% glycerol.

Purity

~95% (SDS-PAGE)

References

1. Wood, Z. A. et al. (2003) Trends Biochem Sci. 28(1):32-40.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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