Preadipocyte Factor1, Mouse, Control Peptide (Pref1, Fetal Antigen 1, FA1, Delta Drosophila homolog-like 1, DLK1, Zona Glomerulosa-specific Factor, ZOG)

Control peptide for P5800-07 (affinity purified antibody). Synthetic peptide consisting of 16aa sequence near the cytoplasmic, C-terminus of mouse Pref-1. Species Sequence Homology: Human, rat: 93%; bovine: 87% Pref-1. Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including Pref-1, involved in lipid metabolism and glucose homeostasis. Preadipocyte factor-1 (Pref-1), an epidermal growth factor-like domain-containing transmembrane protein is implicated in inhibiting preadipocytes differentiation. Pref-1 (mouse 385aa, rat 383aa, human 383aa, chromosome 14q32), also known as fetal antigen 1 (FA1), Delta Drosophila homolog-like 1 (DLK1) or Zona glomerulosa-specific factor (ZOG), is synthesized as a membrane protein in preadipocytes but it is not detectable in mature adipocytes. Dexamethasone, a promoter of adipocyte differentiation, down regulates Pref-1. Pref-1 is proteolytically cleaved to generate a biologically active 50kD ectodomain (24-303aa) or soluble form. Constitutive expression of Pref-1 or addition of its ectodomain inhibits adipogenesis. There are at least four major isoforms (45-60kD) of Pref-1 generated by alternatively splicing. Pref-1/DLK is expressed in tumors with neuroendocrine features, such as neuroblastoma, pheochromocytoma, and a subset of small cell lung carcinoma cell lines. Its expression in normal tissues is restricted to the adrenal gland and placenta. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: ELISA: 10-100ng of control peptide/well Antibody blocking: 5-10ug per 1ug of P5800-05. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.