The B-Bridge Pregnanediol-3-Glucuronide (PDG) Immunoassay kit uses a specifically generated antibody to measure PDG and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure PDG present in diluted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An PDG-peroxidase conjugate is added to the standards and samples. The binding reaction is initiated by the addition of a polyclonal antibody to PDG. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the plate is read at 450nm.
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