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Prolactin (PRL) ELISA Kit

Prolactin (PRL) ELISA Kit

Cat no: ABIN1873398

Supplier: Antibodies-online
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Prolactin (PRL) ELISA Kit: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to prolactin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled prolactin and unlabeled prolactin (Standards or samples) with the pre-coated antibody specific to prolactin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of prolactin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of prolactin in the sample.
Catalogue number: ABIN1873398
Size: 96 tests
Accession: Q28632
Gene: 100009394
Antigen: Prolactin (PRL)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 2.47-200 ng/mL
Sample type: Serum,Plasma,Biological Fluids
Entrez gene: 100009394

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