Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2) ELISA Kit

Cat no: ABIN419896

Supplier: Antibodies-online

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Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN419896
Size:	96 tests
Accession:	O15460
Gene:	8974
Antigen:	P4Ha2
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	0.156-10 ng/mL
Sample type:	Tissue Homogenate,Biological Fluids
Entrez gene:	8974

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Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2) ELISA Kit

Cat no: ABIN419896

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