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Prostaglandin F1 alpha, 6-keto (PGF1a)

Cat no: P9053-32A

Prostaglandin F1 alpha, 6-keto (PGF1a)

Applications:\nSuitable for use in Radio Immunoassay. Other applications not tested. \n\nThaw antiserum at room temperature. Make a 1:25 working dilution of the reconstituted antiserum in PBS plus 0.1% gelatin (i.e. assay buffer). This permits addition of 200ul per assay tube.\n\nSensitivity:\nThe suggested dilution will result in >30% binding of tracer in a liquid phase RIA with 0.2ml of first antibody added to a final volume of 1ml.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9053-32A

Size

1ml

Applications

ELISA

Hosts

Sheep

Form

Supplied in PBS with 0.1% sodium azide.

P Type

Pab

Isotype

IgG

References

1. J.L. Fahey, et al., Amer. Soc. Microbiol. pp. 685-691 (1986)2. W.S. Powell, Methods in Enzymology 86;467-476.

Additional Info

Hapten Reactivity (%)\n\nPGF2alpha 2.10\n6-keto PGF1alpha 100.00\nthromboxane B2 0.07\n13,14-dihydro-15-keto PG2alpha 0.03\n13,14-dihydro-6.15-diketo PGF2alpha 0.02\nPGE2 0.05\nPGD2 0.03\nPGE1 0.06\nPGE2 0.05\n6-ketoPGE1 8.60\nPGF1alpha 7.90\narachidonic acid 0.002

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