Protease Activity BioAssay(TM) Kit

Supplier:

United States Biological

Cat no: P9055-03

Protease Activity BioAssay(TM) Kit

Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the aa sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway.\n\nSample Type:\nCell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food samples, etc.\n\nIntended Use:\nProtease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample.\n\nSensitivity:\n<500pg/well\n\nTest Principle:\nThe assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/EmH5/530nm. The kit is supplied with Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect (same/less than)500pg/well Trypsin present in the sample.\n\nKit Components:\nProtease Assay Buffer: 1x25ml\nProtease Substrate (lyophilized): 1x1vial\nFITC Standard (25uM): 1x200ul\nPositive Control (lyophilized): 1x1vial\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9055-03

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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