Protease Inhibitor Cocktail

Supplier:

United States Biological

Cat no: P9070

Protease Inhibitor Cocktail

Applications: \nUsed generally to inhibit proteases (serine, cysteine, aspartic and metalloproteases). \n\nOne bottle is suf?cient to inhibit proteases present in 20g of cellular extract. Note that some organisms contain higher levels of proteases and may require a higher concentration of protease cocktail to inhibit the intrinsic proteolytic activity. \n\nActivity:\nOne ml of the cocktail will inhibit proteolytic activity equivalent to 1mg of USP pancreatin.\n\nInhibitor Targets:\nAEBSF: Serine proteases, such as trypsin and chymotrypsin\nEDTA Metalloproteases\nBestatin: Aminopeptidases, such as leucine aminopeptidase and alanyl aminopeptidase\nE-64: Cysteine and thiol proteases, such as calpain, papain, cathepsin B and cathespin L\nLeupeptin: Serine and thiol proteases, such as calpain, trypsin, papain and cathepsin B Aprotinin: Serine proteases such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase and kallikrein. Inhibits leukocyte elastase but not pancreatic elastase\n\nReconstitution: \nDissolve powder in 10ml of RIPA lysis buffer (10X) and transfer to a larger container to prepare 100ml final volume. The powder may be prepared and stored as a more concentrated stock for further dilution.\n\nStorage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 1 week at 4C.

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SPECIFICATIONS

Catalog Number

P9070

Size

1Vial

Form

Supplied as lyophilized powder containing 2mM AEBSF, 1mM EDTA, 130uM Bestatin, 14uM E-64, 1uM Leupeptin, 0.3uM Aprotinin when reconstituted in 100ml of buffer.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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