A widely used fluorogenic substrate for 20S proteasome, calpains and other chymotrypsin-like proteases. Cleavage of this AMC peptide by the enzymes generates strongly fluorescent AMC that is monitored fluorometrically.
Sequence:
Suc-LLVY-AMC
Solubility:
DMSO (~5mM)
Molecular Weight:
~763.88kD
Lambda Absorption: 340-360nm
Lambda Emission: 440-460nm
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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