Protein A/G/L, Recombinant

Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains 6x His-tag on the N-terminus with five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody. \n\nProtein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.\n\nSource: E. coli\n\nEndotoxin level: (same/less than)0.1EU/mg\n\nTHEORETICAL PI: 4.76\n\nSPECIFICITY: Under optimal conditions, 1mg protein A/G/L will bind approximately 5-15mg human IgG. Optimal binding of Protein A/G/L to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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