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Protein A/G, Recombinant (Biotin)

Cat no: P9102-17

Protein A/G, Recombinant (Biotin)

Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. \n\nProtein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G. \n\nProtein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.\n\nSPECIFICITY: Under optimal conditions, 1mg protein A/G will bind approximately 5mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-17

Size

100ug

Conjugates

Biotin

Form

Supplied as a lyophilized powder. Labeled with Biotin. Reconstitute with 20ul sterile ddH2O.

Purity

(same/more than)98% (SDS-PAGE and HPLC)\nEndotoxin level: (same/less than)0.1EU/mg

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