Amino acid 32-327 of the Staphylococcus aureus subsp. aureus.
The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding.
The 6x His-tag on N-terminus can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Extinction Coefficient: 7450 (at 280nm)
Specificity:
Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from bovine, guinea pig, hamster, equine, porcine and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
BINDING Capacity: (same/more than)95%
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Stable for 6 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
