The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding.
The 6x His-tag on N-terminus can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Source: E.coli
Endotoxin level: (same/less than)0.1EU/mg
Theoretical PI: 5.56
Specificity: Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from bovine, guinea pig, hamster, equine, porcine, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
BINDING Capacity: (same/more than)95%
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 6 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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