Protein A, Recombinant, Staphylococcus aureus

Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E. coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).\n\nRecombinant Staphylococal Protein A is a non-glycosylated, Polypeptide chain containing 178 amino acids and having a molecular mass of 23,307D.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Protein A.\n\nActivity:\nGreater than 95% binding activity to human IgG.\n\nReconstitution:\nReconstitute the lyophilized powder in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-19

Size

10mg

Form

Supplied as a lyophilized powder. No additives.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Herpes simplex type 1-induced Fc receptor binds to the Cgamma2-Cgamma3 interface region of IgG in the area that binds staphylococcal protein A. Johansson PJ, Nardella FA, Schroder AK, Immunology 1989 Jan;66(1):8-13 2. Staphylococcal protein A inflames the lungs. Normark BH, No rmark S, Nat Med 2004 Aug;10(8):780-1 3. Use of staphylococcal protein A in the analysis of teleost immunoglobulin structural diversity. Bromage ES, Ye J, Kaattari IM, Dev Comp Immunol 2004 Jun;28(7-8):803-14 4. Microsecond folding dynamics of the F13W G29A mutant of the B domain of staphylococcal protein A by laser-induced temperature jump. Dimitriadis G, Drysdale A, Arora P, Proc Natl Acad Sci U S A 2004 Mar 16;101(11):3809-14 5. Validation of helical tilt angles in the solution NMR structure of the Z domain of Staphylococcal protein A by combined analysis of residual dipolar coupling and NOE data. Zheng D, Aramini JM, Protein Sci 2004 Feb;13(2):549-54 6. Atomically detailed folding simulation of the B domain of staphylococcal protein A from random structures. Vila JA, Ripoll DR, Proc Natl Acad Sci U S A 2003 Dec 9;100(25):14812-6.