Protein Gene Product 9.5, Recombinant, Human (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)

PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Protein gene product (PGP9.5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kD was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibres at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. \n\nRecombinant PGP9.5 shows a band at 36kD on SDS-PAGE commassie blue stained gel.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of human PGP-9.5.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-72A

Size

2ug

Form

Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

(same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells. Otsuki T, Yata K, Hyodoh F, Hatada S, Br J Haematol 2004 Nov;127(3):292-8 2. Granular cell tumor: immunohistochemical assessment of inhibin-alpha, protein gene product 9.5, S100 protein, CD68, and Ki-67 proliferative index with clinical correlation. Le BH, Boyer PJ, Kapadia SB, Arch Pathol Lab Med 2004 Jul;128(7):771-5 3. Demonstration of substance P, calcitonin gene-related peptide, and protein gene product 9.5 containing nerve fibers in human cervical facet joint capsules. Kallakuri S, Singh A, Cavanaugh JM, Spine 2004 Jun 1;29(11):1182-6 4. Expression of protein gene product 9.5 in lepromatous eyes showing ciliary body nerve damage and a \" dying back \" phenomenon in the posterior ciliary nerves. Ebenezer GJ, Daniel E, Br J Ophthalmol 2004 Feb;88(2):178-81 5. Distribution of protein gene product 9.5-immunopositive and NADPH-diaphorase-positive neurons in the common marmoset (Callithrix jacchus) accessory olfactory bulb. Nakajima T, Tanioka Y, J Vet Med Sci 2003 Dec;65(12):1307-11 6. Immunoelectron microscopic studies on protein gene product 9.5 and calcitonin gene-related peptide in vallate taste cells and related nerves in the guinea pig. Huang YJ, Wu YH, Microsc Res Tech 2003 Dec 1;62(5):383-95.