Shotgun proteomics, also known as shotgun method, refers to the utilization of bottom-up proteomics techniques to study all proteins present in complex mixtures such as serum, urine, and cell lysates. It combines high-performance liquid chromatography (HPLC) and mass spectrometry (MS). The uniqueness of shotgun proteomics lies in its ability to identify and quantify multiple proteins while minimally separating them.
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