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Protein Kinase A, catalytic subunit, Recombinant (PKA)

Cat no: P9102-91F

Protein Kinase A, catalytic subunit, Recombinant (PKA)

Recombinant full-length human PKA, catalytic subunit alpha, without a His-tag, expressed in E. coli.\n\nSpecific Activity: 8580U/mg where 1 unit of PKA activity equals 1nmol of phosphate incorporated into 30um Kemptide per minute at 30 degrees C with a final ATP concentration of 100um.\n\nApplications:\nSuitable for use in Kinase Assay and MS Tryptic fingerprint. Other applications not tested.\n\nRecommended Dilutions:\nKinase Assay: 0.1-1.8ng phosphorylated 30uM of Kemptide.\nMS Tryptic Fingerprint: Confirmed identity as PKA with the translated sequence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. \n\n

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SPECIFICATIONS

Catalog Number

P9102-91F

Size

5ug

Form

Supplied as a liquid in 30mM potassium phosphate, pH 7.4, 150mM KCl, 1mM EDTA, 1mM DTT, 50% glycerol.

Purity

~90%

References

1. Yonemoto, W.M., et al., Methods Enzymol. 200: 581

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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