Protein Kinase C, delta (PKCd)

Protein kinase C (PKC) mediates a wide range of biological responses, including serotonin release from platelets, histamine release from mast cells, glycogen hydrolysis in the liver, fat synthesis by adipocytes, and many others. PKC was described as a serine/threonine protein kinase activated by the presence of Ca2+, phospholipids, and sn-1,2 diacylglycerol (Nishizuka, 1986). In humans, PKC is found as 11 different isoforms which vary in amino acid sequence, structure, subcellular localization, tissue distribution, substrate specificity, and responses to extracellular signals.\n\nSynthetic peptide corresponding to amino acid residues 658-676 (SAFAGFSFVNPKFELLED) (linear) of the C terminal region of human PKCdelta.\n\nCorresponding antibody P9103-19C recognizes the group 2 PKC isoform PKC delta, the most abundant PKC isoform expressed in hematopoietic tissues. Species Crossreactivity: Human. The antibody is expected to react with most mammalian species based on known homologies.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n

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SPECIFICATIONS

Catalog Number

P9103-19C1

Size

1mg

Applications

ELISA, WB

Reactivities

Hum

Form

Supplied as a lyophilized powder.

Purity

Highly purified

References

1. Kent, J.D., S. Sergeant, D.J. Burns and L.C. McPhail (1996) Identification and regulation of protein kinase C-delta in human neutrophils. J. Immunol. 157(10):4641-4647. 2. Li, W., J.C. Yu, P. Michieli, J.F. Beeler, N. Ellmore, M.A. Heidaran, and J.H. Pierce (1994) Stimulation of the platelet-derived growth factor beta receptor signaling pathway activates protein kinase C-delta. Mol. Cell. Biol. 14(10):6727-6735. 3. Mischak, H., J.H. Pierce, J. Goodnight, M.G. Kazanietz, P.M. Blumberg, and J.F. Mushinski (1993) Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. J. Biol. Chem. 268(27):20110-20115. 4. Nishizuka, Y. (1986) Studies and perspectives of protein kinase C. Science 233:305-312. 5. Wetsel, W.C., W.A. Khan, I. Merchenthaler, H. Rivera, A.E. Halpern, H.M. Phung, A. Negro-Vilar, and Y.A. Hannun (1992) Tissue and cellular distribution of the extended family of protein kinase C isoenzymes. J. Cell. Biol. 117(1):121-133.