Protein Kinase C, gamma, Recombinant, Human (PKCg)

Recombinant Human PKC-gamma produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80kD. PKC-g is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.\n\nUnit Definition: \n~6000-8000 units/mg. One unit of PKC-gamma activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30 degrees C. Recombinant human active PKC-g also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3beta. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.\n\nStorage: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

P9103-25E3

Size

2ug

Form

Supplied as a liquid in 10mM Tris, pH 7.4, 0.1M sodium chloride, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Deficits in the mid-brain raphe nuclei and striatum of the AS/AGU rat, a protein kinase C-gamma mutant.\n\nEur J Neurosci 2005 Dec;22(11):2792-8\n\n2. Protein kinase C gamma mutations in spinocerebellar ataxia 14 increase kinase activity and alter membrane targeting.\n\nBrain 2005 Feb;128(Pt 2):436-42\n\n3. Molecular interaction model for the C1B domain of protein kinase C-gamma in the complex with its activator phorbol-12-myristate-13-acetate in water solution and lipid bilayer.\n\nJ Med Chem 2004 Dec 16;47(26):6547-55\n\n4. Acute restraint stress reduces protein kinase C gamma in the hippocampus of C57BL/6 but not DBA/2 mice.\n\nNeurosci Lett 2004 Sep 30;368(3):293-6\n\n5. Mutation in the catalytic domain of protein kinase C gamma and extension of the phenotype associated with spinocerebellar ataxia type 14.\n\nArch Neurol 2004 Aug;61(8):1242-8\n\n6. Spatial learning in rats is impaired by microinfusions of protein kinase C-gamma antisense oligodeoxynucleotide within the nucleus accumbens.\n\nNeurobiol Learn Mem 2004 Mar;81(2):120-36