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Protein Kinase C iota, active, Recombinant, Human (PKCi)

Cat no: P9103-70J

Protein Kinase C iota, active, Recombinant, Human (PKCi)

N-terminal His6-tagged, recombinant full-length human PKC iota expressed in Sf21 cells.\n\nSpecific Activity: \n~800U/mg, where one unit of PKC iota activity is defined as 1nmol phosphate incorporated into 50uM PKCe Substrate Peptide (ERMRPRKRQGSVRRRV) per minute at 30 degrees C with a final ATP concentration of 100uM.\n\nApplications: Suitable for use in Kinase Assays.\nOther applications have not been tested. \n\n\nMS Tyrptic Fingerprint: Confirmed identity as PKC iota with 43% amino acid coverage of the translated sequence.\n\nRecommended Dilutions: Kinase Assay: 1.6-4.4ng of this lot of enzyme phosphorylated 50uM PKCtide Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.\n

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SPECIFICATIONS

Catalog Number

P9103-70J

Size

10ug

Form

Supplied as a liquid in 20mM HEPES/NaOH, pH 7.4, 250mM sodium chloride, 50% glycerol, 5mM DTT, 2mM EDTA, 2mM EGTA, 0.05% Triton X-100.

Purity

~80% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.

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