Protein Kinase C, iota, lambda, phosphorylated (Thr555/Thr563) (PKCi, PKC, Atypical Protein Kinase C lambda/iota, aPKC lambda iota, PRKCL, DXS1179E, MGC26534)

Protein Kinase C, iota (PKCi) is a 76kD member of the atypical group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. Phosphorylation of PKCi plays a key role in protein trafficking. Phosphorylated PKCi is implicated in high glucose-induced T type II transforming growth factor-beta activity, TNF-a-stimulated NF-kB activation and Bcr/Ablmediated anti-apoptotic effect.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cells treated with PMA, a phorbol ester.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9103-70B

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Tisdale, E.J. et al. (2003) Atypical protein kinase C plays a critical role in protein transport from Pre-Golgi intermediates. J. Biol. Chem. [Jul 17 epub]. 2. Chuang, L.Y. et al. (2003) Regulations of type II transforming growth factor-beta receptors by protein kinase C iota. Biochem. J. [Jul 4 epub]. 3. Standaert, M.L. et al. (2002) Skeletal muscle insulin resistance in obesity-associated type 2 diabetes in monkeys is linked to a defect in insulin activation of protein kinase C-zeta/lambda/iota. Diabetes 51(10):2936-2943. 4. Selzer, E. et al. (2002) Protein kinase C isoforms in normal and transformed cells of the melanocytic lineage. Melanoma Res. 12(3):201-209. 5. Acevedo-Duncan, M. et al. (2002) Human glioma PKC-iota and PKC-betaII phosphorylate cyclindependent kinase activating kinase during the cell cycle. Cell Prolif. 35(1):23-36. 6. Xie, J. et al. (2000) Protein kinase C iota protects neural cells against apoptosis induced by amyloid beta-peptide. Brain Res. Mol. Brain Res. 82(1-2):107-113. 7. Jamieson, L. et al. (1999) Protein kinase Ciota activity is necessary for Bcr-Abl-mediated resistance to drug-induced apoptosis. J. Biol. Chem. 274(7):3927-3930.

Additional Info

Recognizes human Protein Kinase C, iota and lambda when phosphorylated at Thr555 and Thr563, respectively. Peptide competition suggests crossreaction with Protein Kinase C, zeta [pT560] (83% homologous) and partial crossreaction with Protein Kinase C, betaI [pS642] (58% homologous) and Protein Kinase C, gamma [pT655] (43% homologous).\nSpecies Crossreactivity: Mouse Protein Kinase C, iota.