Protein Kinase C, theta, Recombinant, Human (PKCt)

Recombinant Human PKC-teta produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-706 and having a molecular mass of 86kD. PKC-t is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.\n\nUnit Definition: \n~1800 units/mg. One unit of PKCt activity transfers 1 nmole of phosphate to a PKC-t pseudosubstrate peptide per minute at 300C using. Recombinant human active PKC-teta also phosphorylates histone, MARCKS, moesin, RasGRP3, and PKC alpha and PKC epsilon pseudosubstrates. PKC-teta is a novel PKC and does not require calcium for activity. Activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. PKC-teta is involved in T-cell activation and T-cell receptor signaling including T-cell mediated activation of NF-kB and AP-1.\n\nStorage: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

P9103-42H

Size

2ug

Form

Supplied as a liquid in 10mM Tris, pH 7.4, 0.1M sodium chloride, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Purity

(same/more than) 85% (SDS-PAGE)

References

1. Regulatory T cells inhibit protein kinase C theta recruitment to the immune synapse of naive T cells with the same antigen specificity.\n\nJ Immunol 2006 May 15;176(10):5779-87\n\n2. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice.\n\nJ Immunol 2006 Mar 1;176(5):2872-9\n\n3. Lipid raft targeting of hematopoietic protein tyrosine phosphatase by protein kinase C theta-mediated phosphorylation.\n\nMol Cell Biol 2006 Mar;26(5):1806-16\n\n4. Protein kinase C theta co-operates with calcineurin in the activation of slow muscle genes in cultured myogenic cells.\n\nJ Cell Physiol 2006 May;207(2):379-88\n\n5. Menin represses JunD transcriptional activity in protein kinase C theta-mediated Nur77 expression.\n\nExp Mol Med 2005 Oct 31;37(5):466-75\n\n6. Protein kinase C-theta is an early survival factor required for differentiation of effector CD8+ T cells.\n\nJ Immunol 2005 Oct 15;175(8):5126-34