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Protein Kinase G1 beta, N-terminal HIS-Tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase)

Cat no: P9102-95R

Protein Kinase G1 beta, N-terminal HIS-Tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase)

Source:\nRecombinant corresponding to full length human PKG1 beta with N-terminal His tag expressed in Baculovirus infected Sf9 cell expression system (NM_006258).\n\nSpecific Activity:\n0.85pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (SER/THR 14) at RT for 1hour.\n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -70 degrees C. Aliquots are stable for at least 12 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-95R

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.

Purity

Purified ~70%

References

1. Bivalacqua T.J. et al., BJU Int. 2007 Jun;99(6):1488-94. 2. Lee J.H. et al., Retrovirology. 2007 Dec 13;4:91.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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