Protein L, Recombinant, Peptostreptococcus Magnus

Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. United States Biological's Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. \n\nThe 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. \n\nProtein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.\n\nSOURCE: E. coli \n\nSEQUENCE: Amino acid 98-462 of the Peptostreptococcus Magnus. Protein L Contains 5 kappa-binding domain with 6x His-tag on N-terminus. Gene Bank Accession Number CAA25612.\n\nEndotoxin level: (same/less than)0.1EU/mg\n\nSpecificity: Under optimal conditions, 1mg protein L will bind approximately 5mg human IgG. Optimal binding of Protein L to antibodies occurs at pH 7.5 over a pH range of 2.5 to 3.0.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

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