Protein MW Marker, Prestained, 6-175kD

Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 6-175kD when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. \n\nRecommended Usage:\n1. Transfer the desired amount of the Prestained Protein Marker to a separate tube.\nFor visualization during electrophoresis:\nMini-gels: use15ul.\nFull size gels: use 30ul.\n2. Heat the Marker to 95-100 degrees C for 3-5 minutes.\n3. Microcentrifuge briefly. Load directly on to the gel. To ensure uniform mobility, load an equal volume of 1X rSDS Reducing Loading Buffer into any unused wesll.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9106-75

Size

70 Blots

Form

Supplied as a liquid in 70mM Tris-HCl, pH 6.8 at 25 degrees C, 33 mM sodium chloride, 1mM Na2EDTA, 2% SDS, 40mM dithiothreitol (DTT), 0.01% phenol red, 10% glycerol.

References

1. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory (1989). 2. Laemmli, U.K., Nature 227: 680-685 (1970).

Additional Info

Apparent\nProtein Source MW (kD)\nMBP-b-galactosidase* E. coli 175.0\nMBP-paramyosin* E. coli 83.0\nGlutamic dehydrogenase bovine liver 62.0\nAldolase rabbit muscle 47.5\nTriosephosphate isomerase rabbit muscle 32.5\nb-Lactoglobulin A bovine milk 25.0\nLysozyme chicken egg white 16.5\nAprotinin bovine lung 6.5\n\n*MBP = maltose-binding protein.\nMBP-b-galactosidase = fusion of MBP and b-galactosidase.\nMBP-paramyosin = fusion of MBP and paramyosin.