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Protein Phosphatase 1, 2A (PP1, PP2A) Toolbox

Cat no: P9107-29

Protein Phosphatase 1, 2A (PP1, PP2A) Toolbox

For the selective in vitro dephosphorylation of proteins\n\nKit Components:\nP9107-29A: PP1,1x2U\nP9107-29B: PP2A, 1x2U\nP9107-29C: PHI-1,1x50ug \nP9107-29D: Okadaic Acid (sodium salt), 1x25ug \nP9107-29E: Calyculin A, 1x25ug \nP9107-29F: Protein Phosphatase Dilution Buffer, 1x1ml \n\nQuantity: The number of assays available varies with application.\n\nUnit Definition: \nOne Unit releases 1nmol of phosphate/min from 15uM phosphorylase A at 30 degrees C. \n\nStorage and Stability: \nStore at -20 degrees C. Stable for 1 year. \nNote: Do not store P9107-29A, PP1 or P9107-29B,PP2A at -70 degrees C.

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SPECIFICATIONS

Catalog Number

P9107-29

Size

1Kit

References

1. Brautigan et al., J. Biol. Chem. 260: 4295, 1985.\n2. Johnson et al., Mol. Endocrinol. 1: 745, 1987.\n3. Eroddi, et al., Biochem. Biophys. Res. Comm. 128: 705, 1985.\n4. Shenolikar and Nairn, Adv. Sec. Messenger Phosphoprotein Res. 23: 1, 1990.\n5. Hagiwara, Cell 70: 105, 1992.\n6. DeFranco et al., Molec. Endocrinol. 5: 1215, 1991.\n7. Chung et al., Science 253: 560, 1991.\n8. Ingebritsen and Cohen, Eur. J. Biochem. 132: 255, 1983.\n9. Usui, et al., J. Biol. Chem. 258: 10455, 1983.\n10. Anderson, et al., Nature 1343: 651, 1991.\n11. Reinhart, et al., J. Neurosci. 11: 1627, 1991.\n12. Scheidtmann, Mole. Cell Biol. 11: 1996, 1991.\n13. Ito, et al. EMBO J. 19: 562, 2000.\n14. Eto, M., et al., J. Biochemistry. 38: 16952-16957, 1999.

Additional Info

For use with the Phosphatase Activity Kit P4071-70 to establish an assay for PP1 and PP2A activity in biological samples, typically cell lysates.

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