logo
logo
Protein Phosphatase 2A, demethylated (PP2A)

Cat no: P9107-32

Protein Phosphatase 2A, demethylated (PP2A)

Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the a subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca 2+ and Mg 2+ , respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases.\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nImmunoprecipitation: Reported to immunoprecipitate unmethylated PP2A, C subunit.1\nWestern Blot: A 1:1000-10,000 detects PP2A, demethylated in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with P9107-32 (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nIncluded Positive Control: \nA0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P9107-32

Size

200ul

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Prc, Rabbit

Form

Supplied as a liquid 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Mab

Purity

Ascites

Isotype

IgG

References

1. Ogris, E., et al., J. Biol. Chem. 274: 14382

Additional Info

Recognizes unmethylated human PP2A, C subunit, Mr 36kD.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...