Protein Phosphatase 2B beta (Calcineurin B, CnB)

Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types. Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle. Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals. It is a heterodimers of two subunits: Calcineurin B/CnB, the 19kD Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61kD catalytic subunit that is highly homologous with PP1 and PP2A. Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts. CnB belongs to the family of EF-hand Ca-binding proteins. Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin. Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway. Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells. The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin. \n\nApplications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, and Neutralization. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 0.05ug/well can detect 1ng calcineurin as determined by an independent laboratory. \nImmunocytochemistry: 5ug/ml \nImmunoprecipitation: 4ug/500ug cell lysate from rabbit brain and rat brain extracts. \nWestern Blot: 0.5ug/ml detect s the calcineurin regulatory subunit (beta) in rabbit brain and rat brain extracts. \nBiological Neutralization: Inhibits Ni 2+ -activated calcineurin.\nOptimal dilution to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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