Protein Phosphatase 2C alpha, Recombinant, Human (PP2Ca, PPM1A, Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A)

Protein phosphatase 2C alpha (PP2Ca) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Ca (aa1-382) was cloned into an E. coli expression vector (BamHI/HindIII site). \nPP2Ca was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques. Recombinant human PP2Ca is a single, non-glycosylated polypeptide chain containing 382aa (containing His tag, T7 gene 10 leader, XpressTM Epitope).\n\nAA Sequence: \nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW\n\nBiological Activity:\n~8000 U/mg\n\nUnit Defenition: \nOne unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37 degrees C using 10mM of substrate.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, add a carrier protein (0.1% HSA or BSA), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..

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SPECIFICATIONS

Catalog Number

P9107-39B

Size

10ug

Form

Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM DTT, 1mM manganese chloride and 20% glycerol.

Purity

(same/more than) 95% as determined by RP-HPLC and SDS-PAGE.

References

1. J. Biol. Chem. 279: 22,715-22,726 (2004). 2. J. Biol. Chem. 278: 14,299-14,305 (2003). 3. Biochem. J. 320: 801-806 (1996). 4. FEBS Lett. 377: 421-425 (1995). 5. Mamm. Genome 5: 655-656 (1994).