MALDI-TOF analysis of peptide mixtures is capable of tolerating moderate amounts of buffers and salts, with peptide fragments predominantly producing singly charged ions, making MALDI-TOF MS an ideal tool for peptide mass mapping. To confirm the theoretical sequence of a protein/polypeptide sample using MALDI-TOF, enzymatic digestion is first performed on the sample, followed by the verification of the peptide mass fingerprinting (PMF) via MALDI MS.
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