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Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human  (PTP1B)

Cat no: P9109-01A

Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)

Full-length (aa1-436) PTP1B with N-terminal GST-tag (GenBank Accession No. M31724)\n\nSpecific Activity: \n~2,000 pmole/min/ug. One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 degrees C\n\nAssay Buffer:\n50mM Hepes, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP.\n\nApplications: \nSuitable for use in enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Other applications not tested.\n\nStorage and Stability: \nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months.For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9109-01A

Size

20ug

Reactivities

Hum

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4mM KCl, 10% glycerol, 18mM glutathione, and 3mM DTT

Purity

(same/more than) 55 % by SDS-PAGE

References

1. Frangioni, et al., Cell 68: 545 (1992). 2. Elchebly, M., et al., Science 283: 1544 (1999).

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