Proteinase-3, Human, BioAssay(TM) ELISA Kit (cANCA, PR3)

The United States Biological Proteinase-3, Human, BioAssay(TM) ELISA Kit is intended for the detection and semi-quantitative determination of antibodies to PR-3 in human serum. The assay is to be used to detect antibodies in a single serum specimen. \n \nProteinase-3 is a lysosomal enzyme that can be found in human neutrophils. There are two subsets of autoantibodies to sample neutrophils, c- ANCA and p-ANCA. The first subtype, c-ANCA, shows cytoplasmic staining by IFA and is diagnostic for Wegener's granulomatosis. The major antigen responsible for the c-ANCA staining is Proteinase-3. The second subtype, p- ANCA, shows perinuclear staining by IFA. The major antigen responsible for the p-ANCA response has been shown to be myeloperoxidase. The ANCA staining patterns are obtained using ethanol-fixed sample neutrophils. Anti- PR-3 autoantibodies have been associated with Wegener's granulomatosis but less often with microscopic polyangiitis. Other antigen specificity's (CAP 57) can be responsible for the c-ANCA pattern. These antigens are generally not diagnostic for PR-3 associated vaculitus diseases.\n \nKit Components:\nEach kit contains the following components in sufficient quantities to perform the number of tests indicated on the package label.\n1. Microtiter Plate: 1x96 wells\n2. Sample Diluent: 1x30ml\n3. Positive Control, High: 1x400ul\n4. Calibrator: 1x400ul\n5. Negative Control: 1x400ul\n6. Positive Control, Low: 1x400ul\n7. IgG, IgA, IgM (HRP) Goat x human: 1x15ml\n8. Wash Buffer, 20X: 1x50ml\n9. Tetramethylbenzidine (TMB): 1x15ml\n10. Stop Solution: 1x15ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9099-05

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

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